Figure 4.
A hit compound obtained from the small-molecule compound library. (A) Chemical structure and substance name of a hit compound. (B) Confirmation of the inhibitory effect of 1S-14279 in the ELISA system. 1S-14279 is added at various concentrations (0.005–50 μM). The IC50 value of 1S-14279 is 0.26 μM (n=3, mean±SEM). (C) Surface plasmon resonance analysis of 1S-14279 binding to SPAK [T233E]. 1S-14279 is applied at the indicated concentrations. Nonspecific binding to the reference cell is subtracted from each sensorgram to obtain the specific-binding responses. 1S-14279 interacts with SPAK in a concentration-dependent manner. RU, resonance unit.