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. 2015 Mar 24;26(7):1489–1502. doi: 10.1681/ASN.2014080837

Figure 2.

Figure 2.

Individual single antigen beads within a single-assay reaction can have significant differences in the density of target antigen affecting maximum MFI. A saturating amount of W6/32 antibody that binds ubiquitously to class I HLAs was mixed with single antigen beads. The unadjusted results are shown, with the fluorescence representative of the density of the target antigen on each bead. Wide variation in density is seen.