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. 2015 Jun 5;13(6):3581–3605. doi: 10.3390/md13063581

Figure 1.

Figure 1

Work flow showing the different steps involved in the extraction and initial bioassay-guided fractionation of H. siliquosa. Fresh algal samples were extracted with MeOH yielding crude methanolic extract E1. Extract E1 was de-proteinized yielding extract E2 and precipitate P1. Extract E2 was eluted through silica using hexane:ethyl acetate yielding extract E3 which was further screened for antimicrobial and antibiofilm activity against a panel of clinically-relevant human pathogens. Extract E3 was further fractionated using flash chromatography and active fractions analyzed using HPLC prior to future purification and structural elucidation.