Figure 2.
LIVE/DEAD staining and CLSM (60×) of (left) untreated 72 h S. aureus (MRSA) ATCC 33593 biofilms and (right) S. aureus ATCC 33593 72 h biofilms following 24 h challenge with H. siliquosa extract E3 (1.25 mg/mL). Treated MRSA ATCC 33593 biofilms appeared mostly dead (red) confirming the antimicrobial activity seen during screening. The X–Y projections of the treated biofilm suggest a good penetration of the antimicrobial compounds within the exopolymeric matrix with a consistent antimicrobial activity throughout all layers of the biofilm.