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. 2015 Jun 5;13(6):3625–3639. doi: 10.3390/md13063625

Figure 4.

Figure 4

LSA-induced PCC requires RAF1 kinase activity but is not dependent on BRAF. (A) Relative PCC induced by LSA (100 nM, 30 min) in WT, Raf1−/− and Braf−/− mouse embryonic fibroblasts (MEFs). Results are the mean ± SD of 3 independent experiments. * p < 0.01; (B) Representative images of PCC induced by LSA in WT, Raf1−/− and Braf−/− MEFs. Arrows indicate cells counted as PCC. DNA is visualized using propidium iodide; (C) Chromatin condensation induced by LSA (100 nM, 30 min) in Raf1−/− MEFs complemented with tetracycline-inducible WT or kinase dead (KD) human RAF1 cDNA. Cells were induced with doxycycline (Dox, 1 μg/mL for 24 h) prior to LSA treatment. Results are mean ± SD of 3 independent experiments. * p < 0.01; (D) Immunoblotting confirming the induction of WT or KD RAF1 by doxycycline.