Fig. 6.

BRA depends on the signalling environment to regulate key developmental genes. (A,B) hESCs transfected with either an empty/control vector or a BRA over-expression vector were differentiated for 36 h, as indicated and samples were collected to perform comparative gene expression analysis by qRT- PCR. (C,D) qRT-PCR of BRA target genes expressed in endoderm/anterior primitive streak (blue) or in mesoderm/posterior primitive streak (red), respectively. hESCs transfected with either an empty vector (dark colours) or a BRA overexpression vector (light colours) were differentiated as indicated in A,B. For each group of germ layer markers, gene expression is presented as fold change over the wild type reference sample (control vector): FLyA for endoderm genes (C) and FLyB for mesoderm genes (D), as indicated on the y axis. Bars indicate s.d. (n=3) (Student's two-tailed t-test: *P<0.05; **P<0.01; n.s., not significant). F, FGF2; Ly, Ly294002; B, BMP4; A, activin A; N, noggin (N); S, SB431542.