Fig. 1.

External genital morphologies and mutant cell fates in Fgfr2 conditional knockout mice. (A,B,E,F) Endoderm fate-mapping in Shh^gfpcre/+; R26R mice demonstrates that Fgfr2^EndoΔ males have an irregular perineal raphe (triangles) and females have a dysmorphic vaginal opening (black arrows). Both sexes have ectopic endodermal cells on the preputial skin (inset) and lack a distal urethral meatus (white arrows). (C,D,G,H) Ectoderm fate-mapping in Msx2-Cre; R26R mice reveals failed prepuce closure (white arrowheads) in Fgfr2^EctoΔ males and severe hypospadias in Fgfr2^EctoΔ males and females. Scale bars: 100 μm.