Fig. 6.

Fgfr2-mediated cell cycle progression and genetic interactions among Fgfr2, Shh and β-catenin signaling. (A-C) Fgfr2^Δ tissue compartments of Fgfr2^EndoΔ and Fgfr2^EctoΔ mutants have significantly fewer cells, decreased mitotic indices, longer G0/G1 phases and longer total cell cycle times relative to littermate controls. (D) qRT-PCR comparing levels of Fgfr2, Shh, Ptch1 and β-catenin in tissue-specific Fgfr2 and Shh^CreERT2/Δ conditional mutants relative to control littermates. Target gene expression was normalized to Gapdh. Error bars indicate s.d. *P≤0.05, **P≤0.005 (Student's t-test).