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. 2015 Jun 15;142(12):2147–2162. doi: 10.1242/dev.121046

Fig. 5.

Fig. 5.

Rab8a deletion impairs Paneth cell maturation. (A) Lysozyme staining showed reduced numbers of lysozyme+ Paneth cells in Rab8a−/− crypts. ***P<0.001. (B) Quantitative RT-PCR showed no significant change in the Paneth cell gene expression signature in Rab8a−/− intestinal tissues. (C) Lysozyme and EdU (1 h) staining of Rab8a−/− organoids detected lysozyme+ Paneth cells in proliferating buds of surviving organoids. (D) TEM showed that the residual Paneth cells in Rab8a−/− crypts exhibit a reduction in the typical number and size of granules. Arrow points to a granule in a Rab8a−/− crypt. (E) An expansion of smooth ER was observed in all remaining Rab8a−/− Paneth cells. The thickness of stacked ER cisternae was significantly expanded in Rab8a−/− (834±46 nm) as compared with Rab8a+/+ (333±7 nm) Paneth cells. Scale bars: 10 µm in A; 15 µm in C; 2 µm in D; 1 µm in E.