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Effect of OPN RNAi on the expression of OPN in ECA-109 cells. RT-qPCR was used for detecting the effect of OPN RNAi on the mRNA expression of OPN (A). The protein level of OPN in ECA-109 cells was detected by western blot (B). Cell proliferation was monitored with MTT assay (C). Statistical analysis was performed using the t-test and one-way ANOVA. *(P<0.05) indicates a significant difference compared with the control group.
