Figure 4.

A: Real-time RT-PCR was performed to detect the mRNA level of TLR-4. GAPDH was used as an internal reference. B: Western blotting was performed to determine the protein level of TLR-4. GAPDH was used as an internal reference. In LPS group, VSMCs were treated with LPS (20 μg/ml). In LPS + HSYA group, VSMCs were treated with LPS (20 μg/ml) and HSYA (10 μM). In Control group, VSMCs were without any treatment. **P<0.01. LPS: lipopolysaccharide; HSYA: Hydroxysafflor Yellow A; TLR-4: Toll-like receptor-4; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.