MiR-29a promoted PCa cell apoptosis. Cells were transfected with MiR-29a or miR-NC for 48 hours, and then, subjected to cell apoptosis (stained with PI and FITC-Annexin V). A. In LNCaP cells, MiR-29a overexpression caused an increase in early and late apoptotic cells, and a decrease in living cells. B. In PC-3 cells, miR-29a overexpression caused a decrease in living cells and an increase in early apoptotic cells. Each experiment was performed in triplicate.