Table 1.
SNP | Primers sequence | Annealing temperature | PCR amplification products | endonuclease |
---|---|---|---|---|
Rs1799998 | Sense: 5’CAGGAGGAGACCCCATGTGAC3’ | 66.7°C | 538 bp | Hae III |
Antisense: 5’CCTCCACCCTGTTCAGCCC3’ |
SNP | Primers sequence | Annealing temperature | PCR amplification products | endonuclease |
---|---|---|---|---|
Rs1799998 | Sense: 5’CAGGAGGAGACCCCATGTGAC3’ | 66.7°C | 538 bp | Hae III |
Antisense: 5’CCTCCACCCTGTTCAGCCC3’ |