Table 2.
Percentage of Cell Populations Surrounding DRG Neurons and Absolute Number of Inflammatory Cells in DRGs
| Animal no. | DRG | SIVp28 |
CD68 |
CD163 |
MAC387 |
BrdU |
CD3 |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| % | Cells/mm2 | % | Cells/mm2 | % | Cells/mm2 | % | Cells/mm2 | % | Cells/mm2 | % | Cell/mm2 | ||
| A01 | M | NA | NA | 13.8 ± 0.7 | 491.5 ± 20.9 | 3.2 ± 0.3 | 130.9 ± 12.2 | 1.1 ± 0.2 | 45.2 ± 10.3 | 0.8 ± 0.1 | 40.5 ± 4.4 | 2.6 ± 0.7 | 101.1 ± 24.0 |
| A02 | M | NA | NA | 12.7 ± 0.6 | 683.2 ± 74.1 | 2.4 ± 0.5 | 70.9 ± 15.8 | 0.9 ± 0.1 | 25.5 ± 4.0 | 0.9 ± 0.2 | 39.5 ± 7.8 | 6.4 ± 0.7 | 191.2 ± 21.1 |
| A03 | M | NA | NA | 15.3 ± 0.8 | 528.9 ± 107.2 | 11.7 ± 1.1 | 524.0 ± 37.5 | 0.7 ± 0.1 | 24.2 ± 3.6 | NA | NA | 7.0 ± 0.5 | 293.5 ± 26.6 |
| A04 | M | NA | NA | 11.8 ± 1.2 | 420.6 ± 64.1 | 5.8 ± 0.7 | 299.7 ± 34.8 | 3.1 ± 0.3 | 146.6 ± 19.6 | NA | NA | 8.2 ± 0.8 | 345.4 ± 27.8 |
| A05 | M | 8.2 ± 1.4 | 317.4 ± 51.6 | 28.1 ± 1.9 | 1342.0 ± 146.9 | 45.5 ± 3.4 | 1540.6 ± 77.6 | 11.1 ± 0.9 | 545.9 ± 44.3 | 4.7 ± 0.7 | 207.5 ± 28.3 | 7.6 ± 1.4 | 252.6 ± 36.0 |
| A06 | M | 5.0 ± 0.7 | 216.1 ± 36.7 | 26.1 ± 1.8 | 1338.3 ± 72.0 | 16.6 ± 0.8 | 566.7 ± 34.4 | 3.9 ± 0.3 | 171.1 ± 11.2 | 2.0 ± 0.1 | 91.0 ± 7.0 | 3.6 ± 0.4 | 134.4 ± 15.4 |
| A07 | M | 1.5 ± 0.2 | 66.8 ± 11.5 | 25.3 ± 0.7 | 1292.0 ± 50.8 | 22.0 ± 4.0 | 898.5 ± 95.9 | 1.7 ± 0.2 | 88.7 ± 9.0 | 3.8 ± 0.3 | 195.2 ± 8.3 | 3.1 ± 0.4 | 122.6 ± 8.9 |
| A08 | M | 1.2 ± 0.3 | 60.1 ± 15.8 | 19.1 ± 1.1 | 814.5 ± 72.0 | 12.0 ± 3.1 | 466.4 ± 124.1 | 3.5 ± 0.5 | 136.2 ± 17.6 | 1.4 ± 0.2 | 54.0 ± 7.8 | 1.7 ± 0.5 | 96.3 ± 23.3 |
| A09 | T | 0.1 ± 0.05 | 6.1 ± 0.1 | 35.4 ± 2.1 | 1237 ± 131.9 | 26.5 ± 1.9 | 1125.1 ± 132.0 | 2.6 ± 0.5 | 129.6 ± 30.7 | 2.4 ± 0.3 | 102.5 ± 10.2 | 6.0 ± 3.0 | 221.3 ± 116.4 |
| L | 1.1 ± 0.4 | 64.3 ± 16.3 | 29.7 ± 1.8 | 1328.9 ± 89.4 | 26.3 ± 1.7 | 1271.6 ± 71.4 | 5.3 ± 076 | 229.1 ± 33.0 | 2.6 ± 0.5 | 124.6 ± 22.8 | 6.0 ± 0.9 | 284.3 ± 46.5 | |
| S | 0.1 ± 0.05 | 28.7 ± 7.3 | 41.4 ± 1.7 | 1853 ± 82.6 | 15.1 ± 1.5 | 604.3 ± 99.0 | 3.9 ± 0.5 | 152.0 ± 21.4 | 2.2 ± 0.5 | 100.6 ± 19.8 | 7.0 ± 1.1 | 263.2 ± 40.4 | |
| A10 | T | 0.0 ± 0.0 | 0.0 ± 0.0 | 32.1 ± 5.2 | 1596.8 ± 217.9 | 28.4 ± 4.1 | 1120.1 ± 103.6 | 3.4 ± 0.9 | 131.4 ± 30.6 | 2.4 ± 0.7 | 79.0 ± 20.4 | 4.0 ± 1.1 | 143.9 ± 51.9 |
| L | 0.2 ± 0.1 | 20.5 ± 5.7 | 28.0 ± 2.4 | 1468.3 ± 171.7 | 36.8 ± 2.8 | 1781.7 ± 174.1 | 2.5 ± 0.3 | 121.0 ± 14.8 | 3.3 ± 0.4 | 162.9 ± 19.8 | 3.3 ± 0.4 | 125.6 ± 18.9 | |
| S | 0.1 ± 0.05 | 6.8 ± 1.1 | 27.4 ± 1.1 | 1420.0 ± 257.1 | 18.2 ± 1.2 | 688.9 ± 104.8 | 2.9 ± 0.3 | 115.9 ± 14.5 | 2.7 ± 0.3 | 117.6 ± 16.4 | 4.2 ± 1.4 | 128.4 ± 43.1 | |
| A11 | T | 5.6 ± 2.8 | 332.7 ± 171.4 | 35.1 ± 2.8 | 1812.1 ± 601.8 | 22.0 ± 2.4 | 1490.8 ± 265.2 | 10.9 ± 5.3 | 415.0 ± 115.3 | 16.3 ± 2.0 | 693.6 ± 112.3 | 32.8 ± 3.0 | 1100.2 ± 173.5 |
| L | 0.9 ± 0.2 | 63.1 ± 11.1 | 47.5 ± 1.4 | 1945.5 ± 84.4 | 36.3 ± 1.6 | 1309.0 ± 79.4 | 4.7 ± 0.4 | 213.1 ± 20.2 | 2.0 ± 0.4 | 75.5 ± 17.3 | 14.5 ± 2.3 | 543.0 ± 80.0 | |
| S | 1.1 ± 0.2 | 35.1 ± 8.2 | 48.7 ± 2.0 | 1605.2 ± 112.2 | 37.5 ± 2.6 | 1475.0 ± 144.3 | 4.3 ± 0.4 | 148.2 ± 10.7 | 2.9 ± 0.6 | 90.1 ± 20.1 | 9.0 ± 1.1 | 345.9 ± 42.5 | |
| A12 | T | 0.8 ± 0.2 | 32.8 ± 8.1 | 30.2 ± 3.0 | 1266.1 ± 12.2 | 16.4 ± 1.4 | 622.6 ± 8.0 | 5.3 ± 1.0 | 183.0 ± 7.1 | 3.8 ± 2.7 | 165.0 ± 12.7 | 14.3 ± 1.9 | 476.8 ± 6.3 |
| L | 1.2 ± 0.3 | 51.3 ± 10.4 | 32.5 ± 1.7 | 1481.7 ± 6.0 | 23.2 ± 1.9 | 876.9 ± 7.2 | 9.1 ± 2.2 | 337.6 ± 18.0 | 9.0 ± 1.5 | 311.0 ± 9.5 | 14.8 ± 1.5 | 507.2 ± 5.9 | |
| S | 2.1 ± 0.4 | 90.0 ± 16.7 | 30.4 ± 2.6 | 1357.4 ± 12.7 | 20.1 ± 1.8 | 765.0 ± 6.7 | 4.3 ± 0.7 | 179.7 ± 4.8 | 5.1 ± 0.6 | 228.4 ± 4.7 | 11.1 ± 2.4 | 405.0 ± 8.0 | |
| A13 | T | 1.2 ± 0.4 | 50.6 ± 10.3 | 20.6 ± 1.7 | 1136.3 ± 94.2 | 37.0 ± 0.9 | 2056.8 ± 75.8 | 3.6 ± 0.7 | 182.7 ± 33.3 | 1.7 ± 0.3 | 97.8 ± 17.6 | 1.9 ± 0.4 | 91.1 ± 14.1 |
| L | 1.0 ± 0.2 | 47.4 ± 7.0 | 14.9 ± 1.5 | 844.0 ± 81.5 | 24.5 ± 2.0 | 1193.6 ± 142.3 | 2.3 ± 0.3 | 144.1 ± 12.0 | 1.9 ± 0.2 | 96.5 ± 8.9 | 1.7 ± 0.3 | 88.8 ± 13.3 | |
| S | 1.2 ± 0.2 | 40.0 ± 6.3 | 24.7 ± 2.6 | 943.1 ± 101.6 | 28.6 ± 1.4 | 1195.7 ± 88.0 | 2.0 ± 0.3 | 79.3 ± 12.1 | 2.9 ± 0.3 | 116.8 ± 11.9 | 2.4 ± 0.3 | 96.7 ± 13.2 | |
| A14 | T | 1.0 ± 0.2 | 40.6 ± 7.6 | 17.6 ± 0.9 | 821.9 ± 38.5 | 36.8 ± 2.0 | 1973.1 ± 149.2 | 5.6 ± 1.5 | 286.1 ± 78.5 | 1.7 ± 0.2 | 91.8 ± 9.2 | 4.2 ± 0.7 | 217.2 ± 34.8 |
| L | 0.7 ± 0.1 | 28.8 ± 6.1 | 15.4 ± 1.7 | 712.2 ± 96.2 | 22.8 ± 2.6 | 950.3 ± 97.3 | 3.3 ± 0.5 | 136.1 ± 18.7 | 2.0 ± 0.3 | 95.8 ± 15.6 | 3.5 ± 0.4 | 152.2 ± 16.4 | |
| S | 1.5 ± 0.3 | 57.6 ± 9.3 | 18.9 ± 1.1 | 805.4 ± 63.8 | 20.8 ± 0.6 | 971.9 ± 40.1 | 3.3 ± 0.5 | 126.6 ± 17.6 | 1.7 ± 0.2 | 84.5 ± 13.7 | 3.8 ± 0.6 | 174.3 ± 30.0 | |
| A15 | L | 0.2 ± 0.1 | 14.5 ± 3.2 | 10.9 ± 0.8 | 753.4 ± 81.1 | 6.3 ± 0.9 | 423.0 ± 69.5 | 2.5 ± 0.2 | 156.7 ± 14.5 | 1.3 ± 0.2 | 95.6 ± 16.8 | 1.2 ± 0.3 | 87.4 ± 24.3 |
| S | 0.4 ± 0.1 | 26.1 ± 4.8 | 13.8 ± 1.9 | 667.3 ± 125.7 | 4.5 ± 0.6 | 274.4 ± 36.0 | 3.9 ± 0.7 | 170.5 ± 26.0 | 1.7 ± 0.3 | 87.4 ± 10.9 | 3.1 ± 0.5 | 175.5 ± 33.6 | |
| A16 | L | 1.1 ± 0.7 | 46.8 ± 24.7 | 19.4 ± 1.7 | 1073.2 ± 98.6 | 39.6 ± 1.2 | 2067.8 ± 74.2 | 3.5 ± 0.4 | 140.7 ± 14.3 | 0.6 ± 0.1 | 28.7 ± 5.0 | 1.4 ± 0.2 | 67.5 ± 11.6 |
| S | 1.3 ± 0.2 | 38.5 ± 6.1 | 21.9 ± 2.3 | 1022.2 ± 92.9 | 39.7 ± 1.4 | 1915.6 ± 117.0 | 4.2 ± 0.5 | 159.3 ± 20.4 | 0.9 ± 0.2 | 36.6 ± 6.4 | 4.4 ± 0.2 | 184.8 ± 5.8 | |
Means ± SEM are shown. The percentage of positive cells was calculated as the number of positively stained cells divided by total satellite cells (total hematoxylin-positive cells) surrounding the DRGs, multiplied by 100. Eight ×20 fields were used for each measurement. The absolute number of cells was calculated as the cells/mm2 in eight separate 0.147-mm2 fields by dividing the number of positive cells in the field by the size of the field examined (for a ×20 field, it was 0.147 mm2).
DRG, dorsal root ganglion; L, lumbar DRG; M, multiple DRGs per block, specific anatomical location was not specified, may include thoracic, lumbar, and/or sacral DRG; NA, not applicable; S, sacral DRG; T, thoracic DRG.