Table 1.
Antibodies, dilutions and antigen-retrieval.
| Antibody | Source | Antigen | Dilution | Antigen retrieval |
|---|---|---|---|---|
| Marmoset | ||||
| Polyclonal rabbit anti Ki67 IgG | NCL-Ki67-p, Novocastra | Prokaryotic recombinant fusion protein corresponding to a 1086 dp Ki67 motif-containing cDNA fragment | 1:2500 | Heat treatment for 40 min at 90°C in 1:10 citrate buffer pH6.0 (DAKO REAL Target Retrieval Solution) |
| Mouse anti-Ki67 | BD Pharm | Immunodominant epitope of human Ki67 protein | 1:300 | See above |
| Polyclonal goat anti-doublecortin (DCX) IgG | sc-8066, Santa Cruz Biotechnology | Epitope mapping at the C-terminus of human doublecortin | 1:300 | Microwave treatment at 600 W for 1.5 min in 1:10 citrate buffer pH6.0 (DAKO REAL Target Retrieval Solution) |
| Polyclonal rabbit anti-Tbr2 | AB23345, Abcam | KLH-conjugated linear peptide corresponding to mouse Tbr2 | 1:1500 | See Ki67 |
| MCM2 | MCM2 (D7611)xP Rabbit mAB, Cell signaling | Synthetic peptide corresponding to amino-terminal residues of human MCM2 | 1:50 | See Ki67 |
| Ascl (Mash1) | AB 38557, abcam | Synthetic peptide at N-terminus of first 100 amino-acids of human achaete-scute homolog 1 conjugated to KLH | 1:50 – 1:500 | See Ki67 |
| Monoclonal mouse anti-calretinin IgG | MAB 1568, Millipore | Recombinant rat calretinin | 1:2000 | See DCX |
| C57BL/6 mouse | ||||
| Mouse anti-Ki67 | BD Pharm | Immunodominant epitope of human Ki67 protein | 1:300 | See Ki67 marmoset |
| Polyclonal goat anti-doublecortin (DCX) IgG | sc-8066, Santa Cruz Biotechnology | Epitope mapping at the C-terminus of human doublecortin | 1:250 | See DCX marmoset |