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. 2015 Jun 29;6:637. doi: 10.3389/fmicb.2015.00637

Figure 1.

Figure 1

High pressure liquid chromatography-electrospray/ion trap/mass spectrometry (HPLC-ESI/IT/MS) base peak chromatograms of (A) Flavobacterium johnsoniae (low abundance glycine lipids at retention time 6.5 min not shown; ornithine lipid structure included with variable fatty acid hydroxy group), and (B) Pseudopedobacter saltans lipid extracts. PE, phosphatidylethanolamine; OL, ornithine lipid; OLHFA, Ornithine lipid with hydroxylated fatty acid; I, I', II, II”, unknown intact polar lipids. Chromatographic separation was performed on a Lichrosphere diol column (250 mm by 2.1 mm; 5-μm particles; Grace Alltech Associates Inc.). Elution was achieved with hexane–2-propanol–formic acid–14.8 M aqueous NH3 (79:20:0.12:0.04, v/v/v/v) (A) and 2-propanol–water–formic acid–14.8 M aqueous NH3 (88:10:0.12:0.04, v/v/v/v) (B) starting at 10% B, followed by a linear increase to 30% B in 10 min, followed by a 20-min hold and a further increase to 65% B at 45 min. The flow rate was 0.2 ml min−1, and the total run time was 60 min, followed by a 20 min re-equilibration period.