Fig. 2.

Schematic of sample mounting and motion. (a) Fluorescence excitation (along $x$ axis) and detection (along $z$ axis) are operated on independent perpendicular light paths where the excitation light sheet and the detection focal plane overlap. (b) The custom-made sample chamber is assembled with silicone bellows and seal rings. The clarified fluorescently labeled brain is mounted on a Teflon cylinder in the center of the watertight chamber filled with clearing solution (c) and can be translated and rotated freely with piezo motors (d). (e) Mounting of a clarified fluorescently labeled brain. The brain is glued onto a coverslip and inserted into an adapter that slides into the Teflon cylinder.