Fig. 6.

Whole mouse brain tomography. Imaging of whole transgenic mouse brain treated with CLARITY and cleared with 2,2′-thiodiethanol 63% imaged with an Olympus, $25\times$ objective. (a) 3-D rendering of a parvalbumin-dTomato brain. (b) 3-D rendering of a stack from PV-dTomato mouse brain, $\text{scale bar}=400\mu \mathrm{m}$. (c) High resolution insert showing axonal projections. $\text{Scalebar}=100\mu \mathrm{m}$. (d) Lateral and axial FWHM of the intensity profiles through single axon. Modified with permission from Ref. 13.