Fig. 7.

(a) Basic geometrical optics of a double-sided illumination light-sheet microscope. AD: achromatic doublet, PBS: polarizing beam splitter, red: objective back focal planes and conjugated telecentric planes, green: image planes, blue: $4f$ telecentric lens system, black: alignment irises. (b) Alignment mirror with drilled hole for light transmission, mount for tip adjustment and adaptor for the Teflon tube. (c) With lateral movement the alignment mirror can be placed such that light is transmitted into the opposing excitation arm. (d) The alignment mirror can be rotated to precisely reflect by 90 deg.