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. 2015 Jun 26;10(6):e0131506. doi: 10.1371/journal.pone.0131506

Fig 1. Expression analyses of GDF-15.

Fig 1

(A) Upregulated transcript levels of GDF-15 in the presence of Dox using real-time qRT-PCR analysis. (B) Western blot analysis of GDF-15 protein expression in total protein lysates (30 μg) of TGF-ß1 stimulated cells (4 h). Expression of GDF-15 is increased when TGFBR2 is expressed (+Dox) in both cell clones but not in the parental Tet-On cell line. TGF-ß1 signaling is indicated by increased pSmad2 levels in the TGFBR2-expressing clones. Addition of cycloheximide (CHX) reduces the expression level of GDF-15. ß-Actin and Smad2 served as a loading control.