Table 2. Differentially expressed and de novo synthesized proteins in HCT116-TGFBR2 clones #5 and #22 using mass spectrometry.
| TGFBR2-deficient | |||||
| Acc a | Description | Mass [Da] | MASCOT Score | Sign. Pep. | Cov b [%] |
| 1B59 | HLA class I histocompatibility antigen, B-59 alpha chain | 40844 | 330/267 | 6/4 | 24/24 |
| ASM | Sphingomyelin phosphodiesterase | 70734 | 33/40 | 1/1 | 1/1 |
| CHIP | E3 ubiquitin-protein ligase CHIP | 35290 | 33/32/51 | 1/0/1 | 3/3/3 |
| DTD2 | Probable D-tyrosyl-tRNA(Tyr) deacylase 2 | 18877 | 47/32 | 0/1 | 11/5 |
| ERP29 | Endoplasmic reticulum resident protein 29 | 29032 | 78/48/44 | 1/0/0 | 12/10/10 |
| HGS | Hepatocyte growth factor-regulated tyrosine kinase substrate | 86708 | 54/43 | 1/1 | 2/1 |
| IDHC | Isocitrate dehydrogenase [NADP] cytoplasmic | 46915 | 100/46 | 2/0 | 9/5 |
| RB6I2 | ELKS/Rab6-interacting/CAST family member 1 | 128236 | 79/67/59 | 0/0/0 | 2/2/1 |
| RBM42 | RNA-binding protein 42 | 50496 | 39/32 | 1/1 | 4/4 |
| T2FA | General transcription factor IIF subunit 1 | 58262 | 50/72 | 1/1 | 3/6 |
| TE2IP | Telomeric repeat-binding factor 2-interacting protein 1 | 44404 | 44/34/43 | 1/1/1 | 2/2/2 |
| TFAM | Transcription factor A, mitochondrial | 29306 | 44/36/41 | 1/1/1 | 4/4/4 |
| TGFBR2-proficient | |||||
| Acc | Description | Mass [Da] | MASCOT Score | Sign. Pep. | Cov [%] |
| CSN3 | COP9 signalosome complex subunit 3 | 48412 | 54/31 | 1/1 | 4/3 |
| DOCK8 | Dedicator of cytokinesis protein 8 | 240886 | 41/50 | 0/1 | 1/1 |
| GDF15 | Growth/differentiation factor 15 | 34632 | 38/46 | 1/0 | 5/10 |
| MYH10 | Myosin-10 | 229827 | 192/46 | 3/0 | 4/1 |
| PP2AA | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform | 36142 | 55/30 | 1/0 | 10/4 |
| SYNE2 | Nesprin-2 | 801817 | 44/67 | 0/2 | 0.2/0.2 |
| TGFR2 | TGF-beta receptor type-2 | 65951 | 61/34/32/80 | 1/1/1/1 | 3/2/2/3 |
| TMEM9 | Transmembrane protein 9 | 21074 | 51/34/42 | 1/1/1 | 4/4/4 |
| UBF1 | Nucleolar transcription factor 1 | 89692 | 66/42 | 0/0 | 5/4 |
Numbers for “score”, “peptides” and “coverage” are separated by slashes and correspond to individual experiments; Proteins listed were identified at least in one of triplicate experiments in each of both cell clones, either solely in absence of TGFBR2 (TGFBR2-deficient) or solely in TGFBR2 expressing cells (TGFBR2-proficient).
Acc, Accession; Sign. Pep., Significant Peptides; Cov, Coverage.
a Proteins identified by the SwissProt 2013_02 database with taxonomy set to human.
b Coverage includes all identified peptides.