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. 2015 Jun 29;5:11736. doi: 10.1038/srep11736

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) 293T cells were transfected with 1 μg of HBV proviral construct, 50 ng BST-2 IHA, 50 ng Vpu-myc plasmids, and siRNA as indicated. BST-2 IHA, Vpu-myc, and siRNA efficiency were detected by Western blotting. (b) HBV antigen expression and release in a) were examined with ELISA, and HBsAg release percentages are shown. (c) 293T cells were transfected with 1 μg of pNL4-3 ΔVpu proviral construct, 50 ng BST-2 IHA, 50 ng Vpu-myc plasmids, and siRNA as indicated. BST-2 IHA, Vpu-myc, siRNA efficiency, pr55Gag, and concentrated released p24 capsid were detected by Western blotting. (d) The titers of the released infectious HIV-1 viruses in c) were quantified and are shown in percentages. **P < 0.01. These experiments were performed three times, and the most representative Western blot is shown.