Table 2.

Results summary for examination of mRNA (Exp 1) and protein (Exp 2) within the nucleus accumbens of rat pups on PD4, 8 or 12 following prenatal exposure to 0, 1 or 2 g/kg EtOH on GD17-20.

Nucleus Accumbens		mRNA				Protein
		PD4	PD8	PD12		PD4	PD8	PD12
MOR	0g/kg 1g/kg 2g/kg	2.62 ± 0.52 2.69 ± 0.36 3.01 ± 0.53	2.33 ± 0.23 2.20 ± 0.18 1.73 ± 0.12	1.60 ± 0.13 1.56 ± 0.06 1.38 ± 0.06	Age: F(2,60)=14.006, p<0.0001 Dose: F(2,60)=0.196, p=.822 Age × Dose: F(4,60)=0.761, p=.555	124858.16 ± 3827.70 107210.42 ± 8433.81 123952.91 ± 3160.56	115867.83 ± 7392.51 115815.40 ± 6803.24 120808.35 ± 6950.47	135626.92 ± 2478.19 127742.67 ± 3277.05 91364.74 ± 4315.04	Age: F(2,68)=0.032, p=.969 Dose: F(2,68)=3.571, p<0.05 Age × Dose: F(4,68)=6.23, p<.0005
POMC / β-endorphin	0g/kg 1g/kg 2g/kg	121.94 ± 41.36 52.91 ± 12.36 214.14 ± 66.49	404.84 ± 124.38 157.24 ± 42.92 118.15 ± 18.24	308.19 ± 54.38 406.20 ± 64.94 385.42 ± 47.08	Age: F(2,58)=11.053, p<0.0001 Dose: F(2,58)=1.066, p=.351 Age × Dose: F(4,58)=3.792, p<0.05	0.112 ± 0.025 0.093 ± 0.017 0.096 ± 0.017	0.082 ± 0.016 0.120 ± 0.019 0.064 ± 0.010	0.070 ± 0.012 0.074 ± 0.012 0.083 ± 0.011	Age: F(2,64)=2.297, p=.109 Dose: F(2,64)=.675, p=.513 Age × Dose: F(4,64)=1.857, p=.129
KOR	0g/kg 1g/kg 2g/kg	2.56 ± 0.29 3.57 ± 0.35 3.44 ± 0.44	3.39 ± 0.44 4.11 ± 0.39 5.92 ± 0.30	4.49 ± 0.28 4.30 ± 0.21 4.04 ± 0.37	Age: F(2,56)=10.577, p<0.0005 Dose: F(2,56)=5.554, p<0.01 Age × Dose: F(4,56)=5.65, p<0.001	100811.18 ± 3915.24 98333.27 ± 3074.02 90815.98 ± 4211.37	96215.92 ± 5799.11 97593.04 ± 2701.47 105432.80 ± 4372.21	104894.08 ± 1299.38 100192.23 ± 2734.94 93432.43 ± 3859.63	Age: F(2,66)=0.631, p=.535 Dose: F(2,66)=0.875, p=.421 Age × Dose: F(4,66)=2.527, p<0.05
PPD / dynorphin	0g/kg 1g/kg 2g/kg	5.04 ± 0.46 7.28 ± 1.20 7.98 ± 0.92	12.72 ± 1.32 12.88 ± 1.34 18.44 ± 2.21	20.98 ± 3.85 21.58 ± 0.82 25.09 ± 3.48	Age: F(2,60)=38.88, p<0.0001 Dose: F(2,60)=3.109, p=052 Age × Dose: F(4,60)=0.315, p=.867	0.968 ± 0.409 1.279 ± 0.310 1.467 ± 0.538	2.287 ± 0.680 0.977 ± 0.283 0.330 ± 0.109	0.689 ± 0.163 0.554 ± 0.283 0.785 ± 0.272	Age: F(2,64)=1.772, p=.178 Dose: F(2,64)=1.134, p=.328 Age × Dose: F(4,64)=2.788, p<0.05
DOR	0g/kg 1g/kg 2g/kg	3.74 ± 0.62 5.92 ± 0.78 6.48 ± 1.22	5.91 ± 0.76 7.50 ± 0.75 7.77 ± 0.75	9.19 ± 1.35 8.01 ± 0.68 9.79 ± 1.68	Age: F(2,59)=9.281, p<0.001 Dose: F(2,59)=2.135, p=.127 Age × Dose: F(4,59)=0.807, p=.523	111173.68 ± 4365.36 96218.83 ± 5441.77 114707.86 ± 6736.15	108291.47 ± 5026.43 118189.99 ± 4077.11 114695.44 ± 5618.89	107695.35 ± 4391.24 105273.05 ± 2840.05 96313.74 ± 2200.25	Age: F(2,65)=3.59, p<0.05 Dose: F(2,65)=0.220, p=.726 Age × Dose: F(4,65)=3.171, p<0.05
PPE / enkephalin	0g/kg 1g/kg 2g/kg	4.63 ± 1.39 5.70 ± 1.25 7.18 ± 0.44	10.78 ± 0.83 10.40 ± 1.93 15.80 ± 1.73	22.64 ± 3.49 25.53 ± 1.72 23.86 ± 4.13	Age: F(2,56)=42.604, p<0.0001 Dose: F(2,56)=1.096, p=.341 Age × Dose: F(4,56)=.570, p=.685	0.039 ± 0.014 0.042 ± 0.009 0.049 ± 0.015	0.053 ± 0.012 0.084 ± 0.019 0.052 ± 0.018	0.147 ± 0.038 0.083 ± 0.026 0.170 ± 0.062	Age: F(2,63)=8.831, p<0.0005 Dose: F(2,63)=.399, p=.672 Age x Dose: F(4,63)1.3063, p=.277

Data represent means and SEM for designated factors. Significant differences from water-intubated controls (i.e., 0 g/kg) are represented by bold font (p<0.05).