Table 3.
Results summary for examination of mRNA (Exp 1) and protein (Exp 2) within the ventral tegmental area of rat pups on PD4, 8 or 12 following prenatal exposure to 0, 1 or 2g/kg EtOH on GD17-20.
| Ventral Tegmental Area | mRNA | Protein | |||||||
|---|---|---|---|---|---|---|---|---|---|
| PD4 | PD8 | PD12 | PD4 | PD8 | PD12 | ||||
| MOR | 0g/kg 1g/kg 2g/kg |
2.46 ± 0.22 3.67 ± 0.47 2.66 ± 0.31 |
2.09 ± 0.23 2.37 ± 0.42 3.33 ± 0.76 |
2.29 ± 0.48 1.81 ± 0.14 2.24 ± 0.37 |
Age: F(2,56)=2.070, p=.136 Dose: F(2,56)=.857, p=.430 Age × Dose: F(4,56)=1.500, p=.215 |
117787.60 ± 4531.00 128330.09 ± 2534.83 113722.84 ± 5417.27 |
111168.49 ± 8221.46 108331.76 ± 6717.11 113217.77 ± 4807.15 |
120219.20 ± 4071.92 125140.32 ± 6091.26 123954.48 ± 4056.12 |
Age: F(2,66)=3.897, p<0.05 Dose: F(2,66)=.514, p=.600 Age × Dose: F(4,66)=.895, p=.472 |
| POMC / β-endorphin | 0g/kg 1g/kg 2g/kg |
3.97 ± 0.80 4.38 ± 0.87 5.63 ± 1.36 |
6.13 ± 1.33 6.27 ± 2.03 9.09 ± 1.20 |
10.35 ± 1.38 11.36 ± 1.69 12.58 ± 1.99 |
Age: F(2,55)=14.759, p<0.0001 Dose: F(2,55)=1.747, p=.184 Age × Dose: F(4,55)=.106, p=.980 |
0.387 ± 0.197 tissue destroyed 0.264 ± 0.097 |
0.975 ± 0.512 0.809 ± 0.343 0.838 ± 0.555 |
0.921 ± 0.525 1.051 ± 0.386 0.959 ± 0.501 |
Age: F(2,28)=2.701, p=.085 Dose: F(2,28)=.085, p=.919 Age × Dose: F(4,28)=.094, p=.984 |
| KOR | 0g/kg 1g/kg 2g/kg |
2.23 ± 0.13 2.32 ± 0.19 2.10 ± 0.21 |
1.90 ± 0.11 2.30 ± 0.21 3.13 ± 0.62 |
1.73 ± 0.16 1.97 ± 0.23 1.58 ± 0.25 |
Age: F(2,55)=4.145, p<0.05 Dose: F(2,55)=.838, p=.438 Age × Dose: F(4,55)=2.085, p=.095 |
94981.68 ± 2480.91 84392.65 ± 3908.53 91051.88 ± 3255.87 |
79846.06 ± 5409.50 78766.42 ± 2952.21 97012.18 ± 5168.83 |
91909.90 ± 2402.59 82291.56 ± 2544.92 78804.42 ± 2781.36 |
Age: F(2,66)=2.687, p=.076 Dose: F(2,66)=4.310, p<0.05 Age × Dose: F(4,66)=5.188, p<0.005 |
| PPD / dynorphin | 0g/kg 1g/kg 2g/kg |
6.33 ± 0.47 6.61 ± 1.03 9.50 ± 0.72 |
8.15 ± 0.54 8.36 ± 0.74 9.68 ± 0.96 |
8.38 ± 1.09 8.20 ± 1.47 11.18 ± 1.81 |
Age: F(2,53)=1.964, p=.150 Dose: F(2,53)=4.759, p<0.05 Age × Dose: F(4,53)=.225, p=.923 |
2.10 ± 1.70 tissue destroyed 0.70 ± 0.40 |
4.80 ± 2.00 6.70 ± 3.30 4.50 ± 2.60 |
5.30 ± 3.10 7.50 ± 4.30 4.60 ± 3.70 |
Age: F(2,28)=2.500, p=.100 Dose: F(2,28)=.188, p=.830 Age × Dose: F(4,28)=.178, p=.948 |
| DOR | 0g/kg 1g/kg 2g/kg |
2.25 ± 0.31 2.82 ± 0.82 2.77 ± 0.59 |
3.93 ± 1.26 4.87 ± 1.33 3.88 ± 0.74 |
2.99 ± 0.28 7.03 ± 1.77 6.49 ± 1.24 |
Age: F(2,55)=4.469, p<0.05 Dose: F(2,55)=1.99, p=.147 Age × Dose: F(4,55)=.825, p=.515 |
64483.55 ± 8041.58 69866.27 ± 5914.94 61269.89 ± 9288.83 |
65229.18 ± 8432.09 58412.89 ± 6015.43 54109.21 ± 5022.88 |
71046.83 ± 6438.47 61659.10 ± 2866.75 67567.03 ± 7230.39 |
Age: F(2,66)=.813, p=.448 Dose: F(2,66)=.508, p=.604 Age × Dose: F(4,66)=.425, p=.790 |
| PPE / enkephalin | 0g/kg 1g/kg 2g/kg |
3.33 ± 0.42 4.01 ± 1.16 7.34 ± 1.79 |
7.30 ± 0.84 6.60 ± 0.46 8.20 ± 1.21 |
6.62 ± 0.64 12.74 ± 2.52 8.01 ± 1.25 |
Age: F(2,53)=6.713, p<0.01 Dose: F(2,53)=2.135, p=.128 Age × Dose: F(4,53)=3.043, p<0.05 |
0.011 ± 0.005 tissue destroyed 0.005 ± 0.003 |
0.028 ± 0.009 0.044 ± 0.023 0.017 ± 0.006 |
0.027 ± 0.010 0.116 ± 0.089 0.058 ± 0.053 |
Age: F(2,28)=2.188, p=.131 Dose: F(2,28)=.668, p=.521 Age × Dose: F(4,28)=.527, p=.717 |
Data represent means and SEM for designated factors. Significant differences from water-intubated controls (i.e., 0g/kg) are represented by bold font (p<0.05).