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. 2015 Mar 18;6(13):10746–10758. doi: 10.18632/oncotarget.3628

Figure 3. CBLC gene silencing causes a homologous recombination defect.

Figure 3

A and B. Bar charts indicating the frequency of cells with greater than five RAD51 (A) and γH2AX (B) nuclear foci in response to exposure to 10 Gy ionising radiation and CBLC siRNA. CBLC siRNA reduced the frequency of cells with RAD51 foci * p<0.05 Student's t test vs. control siRNA transfected cells at the same time point. Data from siBRCA2 transfected cells is shown as a positive control. C. Bar chart indicating GFP signal generated by repair of the DR-GFP DNA substrate. Data shown is normalised to the % of GFP positive cells in a mock-transfected sample. CBLC siRNA reduced the frequency of GFP+ve cells *p<0.05 Student's t test vs. control siRNA transfected cells. Data from siBRCA2 transfected cells is shown as a positive control. Where error bars are shown these represent the standard error of the mean (SEM) from three independent experiments.