Skip to main content
. 2015 Mar 25;6(13):10840–10852. doi: 10.18632/oncotarget.3450

Figure 4. Interaction between HOTTIP, WDR5 and MLL1 on gene expression in Panc1 cells.

Figure 4

(A) Cells were transfected with siHOTTIP, siMLL, siEZH2 or siCtrl, and analyzed by microarrays for changes in gene expression. Regulation of the same genes is illustrated in the Venn diagram. Panc1 cells were transfected with siHOTTIP or siCtrl and genes coregulated by HOTTIP or MLL1 were analyzed by real time PCR. (B) Panc1 cells were transfected with siAURKA or siCtrl, and effects on cell growth and cell cycle progression were determined as outlined in the Materials and Methods. (C) Effects of Annexin V staining and PARP cleavage and (D) cell migration in a scratch assay were determined as Materials and Methods. (E) Panc1 cells were transfected with siHOTTIP, siMLL1 and siWDR5 or siCtrl and analyzed by Western blots as outlined in the Materials and Methods. Results are expressed as means ± SD for 3 replicated determinations, and significant (p < 0.05) change is indicated (*).