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. 2015 Mar 14;6(13):11047–11060. doi: 10.18632/oncotarget.3567

Figure 3. Age-dependent demethylation of aberrantly methylated CGIs.

Figure 3

DNA methylation (arbitrary units) of the 18 tested CGIs at 9 and 12 months of age in the Mdr2-KO (grey) and Mdr2−/+ (white) liver. Fifteen of these 18 genes (“A” and “B” groups) were methylated in 9-month-old mice in both the Mdr2-KO and Mdr2−/+ liver, while at the late precancerous stage, in 12-month-old mice, they were specifically demethylated in the control Mdr2−/+ liver. An additional two genes (“C”) were hypermethylated at both stages in Mdr2-KO only, and one gene (“D”) was hypermethylated in Mdr2-KO compared to the Mdr2−/+ liver at the late precancerous stage only. MSRE-PCR quantified using the ScionImage software and normalized to CryaA; 3 males per group.