Figure 6. Pygo2 regulates E-cadherin expression not noly via binding to and promoting its promoter methylation directly, but also by up-regulating zeb2 indirectly.

(A) E-cadherin protein level was increased accompany by the addition with 5-azacytidine in Huh7 cells, compared with lanes 1, 3 and 5. Treatment of Huh7-overexpression Pygo2 cells with 5Aza partially restored expression of E-cadherin, compared with lanes 2, 4 and 6. (B) MSP assay showed that Pygo2 could promote E-cadherin promoter CpG island methylation. (C) Bisulfite sequencing PCR assay confirmed that Pygo2 could promote E-cadherin promoter methylation. (D) Zeb2 protein and mRNA level were decreased in 97H-shPygo2 cells compared with Ctrl cells. (E) Zeb2 protein and mRNA level were increased when Pygo2 was over-expressed in Huh7 cells. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.