Table 1. Primers sequences.

RT-PCR	Primer name	F:5′-3′	R:5′-3′
	Pygo2	CCTTCTCTGTCCCAACGATTT	CCAGGAAAGGGACTTGTGTTAG
	E-Cadherin	AGCCATGTACGTTGCTATCC	CGTAGCACAGCTTCTCCTTAAT
	β-actin	ATAGCACAGCCTGGATAGCAACGTAC	CACCTTCTACAATGAGCTGCGTGTG
	Zeb2	CCCATTCTGGTTCCTACAGTTC	GGGAAGAACCCGTCTTGATATT
	Slug	AACTACAGCGAACTGGACAC	GAGGATCTCTGGTTGTGGTATG
	Snail	CAGATGAGGACAGTGGGAAAG	GAGACTGAAGTAGAGGAGAAGGA
	Kras	CAAGAGTGCCTTGACGATACA	GACCTGCTGTGTCGAGAATATC
shRNA	Pygo2-1	GATCCGCCTGCATACTCACATCTGAT TCAAGA-GATCAGATGTGAGTATGCAG GTTTTTTACGCGTG	AATTCACGCGTAAAAAACCTGCATAC TCACATCTGATCTCTTGAATCAGATGT GAGTATGCAGGCG
	Pygo2-2	GATCCGCTCACATCTGACGGAGTTTTTC AAGAGAAAACTCCGTCAGATGTGAGTT TTTTACGCGTG	AATTCACGCGTAAAAAACTCACATCTG ACGGAGTTTTCTCTTGAAAAACTCCGT CAGATGTGAGCG
	E-Cadherin	GATCCGCACCAAAGTCACGCTGAATTTC AAGAGAATTCAGCGTGACTTTGGTGTTT TTTACGCGTG	AATTCACGCGTAAAAAACACCAAAGTC ACGCTGAATTCTCTTGAAATTCAGCGTGA CTTTGGTGCG
Pbobi-cmv	Pygo2	GCTCTAGAATGGATTACAAGGATGACGA CGATAAGGCCGCCTCGGCGCCGCCCCCA	CCCTCGAGTCACCCATCGTTAGCAGCC AC
Chip-RT-PCR	E-Cadherin-P1	CAGTTGCTATGATGAGCCAAGA	GGGAAGTCAGTGTTCTCCTTTG
	E-Cadherin-P2	CTCTCATTGGCCTCAATCTCTC	GCCACTGACCAGCTCATTTA
	E-Cadherin-P3	ACCACGCCTGGCTAATTT	GATCACGAGGTCAGGAGATTG
	E-Cadherin-P4	CTCACTAACCCATGAAGCTCTAC	GCCGAGGCTGATCTCAAAT
	E-Cadherin-P5	CACCTGTACTCCCAGCTACTA	GGTCTCACTCTTTCACCCAAG
Methylation	MSP for E-Cadherin Promoter-M	TGGGTAAGATAGAGCGAGATTTC	TCTCGAACTCCTAAACTAAAACGAT
	MSP for E-Cadherin Promoter-U	GGGTAAGATAGAGTGAGATTTTGT	TCTCAAACTCCTAAACTAAAACAAT
	BSP for E-Cadherin Promoter	TTTTAGTAATTTTAGGTTAGAGGGTTAT	AAACTCACAAATACTTTACAATTCC