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. 2015 Mar 14;6(13):11125–11138. doi: 10.18632/oncotarget.3575

Figure 2. The effect of Rab3D on tumor cells morphology and ruffle formation.

Figure 2

(A). The effect of Rab3D on cytoskeletal rearrangements. Control and siRab3D MDA-MB-231 cells were kept in serum and then fixed. Membrane ruffles formation was analyzed by confocal microscope using a Rhodamine labeled phalloidin antibody to visualize actin cytoskeleton. Scale bar, 50 μm. (B). The effect of overexpressing Rab3D on tumor cell morphology. MCF-7 cells transiently transfected with GFP or GFP-Rab3D-expressing plasmid. Nuclei were stained with DAPI and actin was stained with Rhodamine labeled phalloidin antibody. (C). The effect of different Rab3D mutants on tumor cell morphology. Rab3D (Green), Actin (Red), DAPI (Blue). Scare: 50 μm.