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. 2015 Mar 14;6(13):11252–11263. doi: 10.18632/oncotarget.3594

Figure 1. Overexpression of E2F1, not its DNA binding deficient mutant, E2F1(E138), enhances glycolysis through repressing SIRT6.

Figure 1

(A) Over-expression of E2F1, but not E2F1(E138), enhanced glucose uptake and lactate production in PC3 and UMUC3 cells. Glucose uptake and lactate production were measured 48 h after transfection. (B) Western blots comparing levels of SIRT6, GLUT1, and PDK1 after over-expression of E2F1 or E2F1 (E138) in PC3 and UMUC3 cells. Tubulin was used as loading control. (C) Over-expression of SIRT6 blocked the enhanced glucose uptake and lactate production induced by E2F1 over-expression in PC3 cell. Three replicates were performed for each experiment. Each column represents the mean and SEM of 3 independent experiments. V: empty vector.*P < 0.05, **P < 0.01, ***P < 0.001.