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. 2015 Mar 14;6(13):11252–11263. doi: 10.18632/oncotarget.3594

Figure 3. Increased E2F1 and its binding to SIRT6 promoter under hypoxia.

Figure 3

(A) Growth curve of PC3 and UMUC3 cells showed continued proliferation under hypoxia (0.2% O2) compared to normoxia. (B) Western blot comparing levels of E2F1, HIF1a and SIRT6 in PC3 and UMUC3 cells grew under normoxia (C) and hypoxia (H). (C) Compared to normoxia (C), SIRT6 mRNA level was significantly decreased after 24 hours of hypoxic exposure (H). (D) Dual reporter luciferase assay comparing promoter activities of wild type and mutated E2F1 binding site under normoxia (black bars) and hypoxia (white bars). (E) ChIP assays demonstrated increased E2F1 binding to SIRT6 promoter region in PC3 and UMUC3 cells following 12 hours of hypoxic exposure compared to normoxia. Three replicates were performed for each experiment. Each column represents the mean and SEM of 3 independent experiments.*P < 0.05, **P < 0.01, ***P < 0.001.