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. 2015 Mar 14;6(13):11252–11263. doi: 10.18632/oncotarget.3594

Figure 4. Knocking down E2F1 increases SIRT6 and decreases glycolysis.

Figure 4

(A) E2F1 silence increased SIRT6 protein and its histone deacetylase activity in UMUC3 cells (left). Increase of SIRT6 mRNA was detected by qRT-PCR in E2F1 siRNA transfected cells (E2F1-KD) compared to untransfected (C) and control siRNA transfected cells (M) (right). (B) Compared to untransfected (C) and control siRNA transfected cells (M), dual reporter luciferase assay showed E2F1 silence (E2F1-KD) increased SIRT6 promoter activity. (C) Compared to control siRNA transfected cells (M), E2F1 knock down (KD) increased SIRT6 and decreased GLUT1 and PDK1 under normoxic and hypoxic (H) conditions in UMUC3 cell. (D) Glucose uptake and lactate production were inhibited after E2F1 knock down under normoxic and hypoxic conditions in UMUC3 cells. M: mock siRNA; H-M: mock siRNA under hypoxia; E2F1-KD: E2F1 knock-down; E2F1-H-KD: E2F1 knock-down under hypoxia. Three replicates were performed for each experiment. Each column represents the mean and SEM of 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.