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. 2015 Mar 14;6(13):11252–11263. doi: 10.18632/oncotarget.3594

Figure 5. HDAC1 forms a complex with E2F1 and up regulated SIRT6 expression.

Figure 5

(A) HDAC1 was immuno-precipitated by anti-E2F1 antibody in PC3 and 5637 cells. (B) HDAC1 knock down (KD) deceased SIRT6 protein in PC3 cell compared to control siRNA transfected cells (M). (C) Western blots of HDAC1, Ac-E2F1, E2F1 and SIRT6 after over-expression of HDAC1 or its mutant (H141A) in PC3 and UMUC3 cells. The HDAC1(H141A) mutant has no deacetylase activity. (D) Over expressing HDAC1, not HDAC1(H141A), increased SIRT6 mRNA level. Three replicates were performed for each experiment. Each column represents the mean and SEM of 3 independent experiments. V: empty vector; M: mock siRNA. *P < 0.05, **P < 0.01, ***P < 0.001.