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. 2015 Mar 14;6(13):11310–11326. doi: 10.18632/oncotarget.3597

Figure 5. Myeloid cell depletion enhances the efficacy of MIS416 immunization in ovarian tumor-bearing mice.

Figure 5

a) Adoptive transfer of OT-I cells was performed in MOSEC-IE9-bearing mice at day 30. Vaccination (MIS416 plus OVA) or control (vehicle plus OVA) treatments were administered on days 31 and 38. Beginning on day 50, mice were administered weekly anti-CD11b mAb or control IgG (isotype) for a maximum of 6 weeks, and monitored for tumor progression requiring euthanasia. Time to euthanasia was displayed by Kaplan-Meier plots (n = 16 mice per group). MIS416 vaccination followed by isotype significantly prolonged time to euthanasia compared to treatment with vehicle followed by isotype (log-rank, p < 0.0001). MIS416 vaccination followed by anti-CD11b mAb treatment led to significantly prolonged survival compared to MIS416 vaccination followed by isotype (p = 0.0013). In the absence of prior MIS416 vaccination, anti-CD11b mAb had no benefit. b) Similarly treated mice (n = 3 per treatment group) were pre-selected for sacrifice at day 59 after tumor challenge, and visible tumor was removed and weighed. MIS416-vaccinated mice had reduced tumor weight compared with non-vaccinated groups (p = 0.004; adjusted p-value for multiple comparisons = 0.01), while anti-CD11b mAb had no significant effect.