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. 2015 Mar 30;6(13):11378–11394. doi: 10.18632/oncotarget.3409

Figure 2. OP9 but not ALL cells generate Galectin-3 containing exosomes.

Figure 2

Western blot analysis on protein lysates of exosomes and the cells that secrete them. A. Expression of the ER marker calreticulin and the exosome marker TSG101 in exosomes and total cell lysates (cells) of OP9 stroma and US7 ALL. B. OP9 or TXL2 ALL cells treated for 48 hours with DMSO (−) or 20 μM exosome secretion inhibitor GW4869 (+). Exo, exosomes from the cells; cells, total lysates of the same cells. 20 μg protein/lane. C. Lysates of cells/exosomes of mouse embryonic fibroblasts (MEFs) from lgals3+/+ (WT) or lgals3 −/− (KO) mice. 20 μg protein/lane. D. Total non-cell associated extracellular (e.c.) Galectin-3 present in 100 μl OP9 stromal-conditioned medium or exosome content equivalent to 100 μl medium. E. Exosomes isolated from OP9 cells held for 24 or 48 hours without FBS examined for Gal3 and TSG101 content using Western blotting.