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. 2015 Mar 20;6(13):11492–11506. doi: 10.18632/oncotarget.3416

Figure 4. MACC1 upregulates TWIST1 and TWIST2 expression in GC cells and tumor tissues.

Figure 4

(A) Western blot analysis (left panel) and quantitation (right panel) of MACC1, TWIST1, and TWIST2 expression in response to overexpression or silencing of MACC1 (oxMACC1 and shMACC1) in BGC-823 cells. *p < 0.05; **p < 0.01, n = 3. WT, wild type. GAPDH was used as a loading control. (B) Representative images (left panel) and quantitation (right panel) of MACC1, TWIST1, and TWIST2 immunostaining in xenograft GC tissues. *p < 0.05; **p < 0.01, n = 6. (C) Nuclear expression of MACC1, TWIST1 and TWIST2 proteins in human primary GC tissues (T) and in matched adjacent non-tumor tissues (N) Lamin was used as the loading control. *p < 0.05; **p < 0.01, n = 5.