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. 2015 Mar 21;6(13):11547–11560. doi: 10.18632/oncotarget.3413

Figure 1. BCL6B expression was silenced by promoter region hypermethylation in HCC.

Figure 1

A. The expression of BCL6B was detected by semi-quantitative RT-PCR before and after 5-Aza treatment in HCC cell lines. GAPDH: internal control. (+): 5-Aza treated, (−):5-Aza untreated; SMMC-7721, HepG2, PLC/PRF5, LAM3, SNU449, Sk-hep1, Bel-7402 and HBXF344 are HCC cell lines B. Methylation specific PCR results of BCL6B gene in human HCC. U: unmethylated allele; M: methylated allele; IVD: in vitro methylated DNA; NL: normal lymphocyte DNA; NI, N2, N3, N4, N5: non-cancerous liver tissue samples: NT1, NT2, NT3, NT4 NT5: non-cancerouse tissue; HCC1, HCC2, HCC3, HCC4, HCC5: primary HCC samples. C. BSSQ results of BCL6B promoter region in HepG2, SNU449, HXBF344, BeL-7402, N1, N2, HCC1, HCC2. MSP: MSP amplification region; Filled circle: methylated CpG sites; opened circle: unmethylated CpG sites; TSS: transcription start site; BSSQ: BSSQ amplification region.