Table 1. Quantitative analysis of immunohistochemical evaluation of Sp1 and Sp3.

Immunohistochemical staining was performed essentially as described in methods. The staining results were scored blindly and semi quantitatively based on staining intensities and proportion of positive staining tumor cells. Briefly, the proportion of positive tumor cells was scored as follows: 0, no stained cells; 1, 1–50%; 2, 50–100% positive staining. The intensity score represents the average staining intensity of tumor cells: +, mild; ++, weak; +++, strong intensity. The statistical significance was calculated using Pearson Chi-Square test.

		Negative	Positive	Total	Pearson Chi-Square
Normal	Sp1	3	0	3	Value 7.995 p = 0.035
Carcinoma		8	27	35
Total		11	27	38
Normal	Sp3	0	0	0	Value 1.327 p = 0.249
Carcinoma		23	11	34
Total		23	11	34