Skip to main content
. 2015 Mar 23;6(13):11640–11651. doi: 10.18632/oncotarget.3449

Figure 1. Cx43 expression is associated with increased malignancy in prostate cancer cells.

Figure 1

(A) Expression of connexin mRNA in prostate cancer cell lines (LNCaP, C4-2, DU145 and PC-3) measured by qRT-PCR. A summary of relative connexin mRNA levels in the indicated cells. Only connexin isoforms with Ct ≤ 35 are included. The color scheme shows qualitative information (blue: low expression; red: high expression). The insert numbers represent the relative levels of each connexin isoform with the median value in each column set to 1. Three replicas were analyzed for each cell line and the average values were used for the table. All the errors were <10% and are not shown for simplicity. GAPDH mRNA expression levels were used as reference in data analysis with the 2-ΔΔCT method. (B) Quantification of CX43 mRNA expression, using the 2−ΔΔCT method with GAPDH mRNA as reference values. The quantity of CX43 mRNA in LNCaP cells was set as 1. n.s., not significant. P values were calculated using one-way ANOVA and Dunnett's post-test. ***P ≤ 0.001. (C) Fold change of CX43 expression from RNA-seq analysis of a mouse prostate cancer model. CX43 expression was up-regulated in metastatic prostate cancer compared to primary cancer in the same mouse. The expression of CX43 in primary tumors was set as reference. **P ≤ 0.01. (D) Cx43, E-Cadherin and N-Cadherin protein levels in prostate cancer cell lines detected by Western blot using their respective antibodies. β-actin serves as loading control.