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. 2015 Mar 23;6(13):11640–11651. doi: 10.18632/oncotarget.3449

Figure 2. Gap-junction-mediated intercellular communication is not required for growth of prostate cancer cells.

Figure 2

(A) Growth of neither LNCaP nor PC-3 cells was affected by pharmacological inhibition of gap-junctions with increasing concentration of CBX. Cell growth was measured by CellTiter-Glo® Luminescent Cell Viability Assay and the units are arbitrary. The experiments were performed in triplicate and representative results are shown. No statistically significance difference was detected. (B) Growth of neither LNCaP nor PC-3 cells was affected by the connexin mimetic peptide ACT-1. The experiments were performed similarly and analyzed as in (A). No statistically significance difference was detected. (C) Gap-junctions that mediate intracellular transfer of calcein AM dye are functional in PC-3 (left panel), but not LNCaP (right panel) cells. Fluorescent (calcein), far-red (DiD) and phase images were captured at the same time (0-4 hours) with a Leica DMI6000B microscope with a 10x objective lens.