Table 1.

Recipe to make two 12% 1 mm SDS-PAGE gels.

Resolving gel, 12% acrylamide			Stacking gel, 4% acrylamide
solution	[Stock]	15 mL	solution	[Stock]	5 mL
H2O		4.23 mL	H2O		3.541 mL
Tris-HCl, pH 8.8	1.5 M	3.75 mL	Tris-HCl, pH 6.8	1 M	625 μL
acrylamide	40%	4.38 mL	acrylamide	40%	487 μL
bis-acrylamide	2%	2.4 mL	bis-acrylamide	2%	267 μL
SDS	20%	75 μL	SDS	20%	25 μL
APS	10%	150 μL	APS	10%	50 μL
TEMED		15 μL	TEMED		5 μL