Table 2.

Recipe to make one 4% 1.5 mm native-PAGE gel.

Resolving gel, 4% acrylamide			Stacking gel, 3% acrylamide
[Final]	[Stock]	10 mL	[Final]	[Stock]	5 mL
H2O		6.337 mL	H2O		2.365 mL
90 mM Tris-borate, pH 8.3	0.9 M	1000 μL	50mM Tris-HCl, pH 6.8	1 M	250 μL
2.5% Sucrose	25%	1000 μL	2.5% sucrose	25%	500 μL
4% acrylamide	40%	974 μL	3% acrylamide	40%	300 μL
bis-acrylamide	2%	519 μL	bis acrylamide	2%	1500 μL
5 mM MgCl2	1 M	50 μL	5mM MgCl2	1 M	25 μL
1mM ATP	1 M	10 μL	1mM ATP	1 M	5 μL
APS	10%	100 μL	APS	10%	50 μL
TEMED		10 μL	TEMED		5 μL