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. Author manuscript; available in PMC: 2015 Jun 29.
Published in final edited form as: Virology. 2006 Feb 28;348(2):462–474. doi: 10.1016/j.virol.2006.01.014

Table 3.

Presteady state kinetics of single nucleotide incorporation

RTs Template-primer
RNA–DNA
DNA–DNA
kpol (s−1) Kd (μM) kpol (s−1) Kd (μM)
WT 38.7 ± 3.53a 5.1 ± 1.97 26.1 ± 2.20 1.6 ± 0.71
K103N 40.2 ± 2.21 4.8 ± 1.07 21.3 ± 1.71 2.6 ± 0.39
G190A 38.1 ± 4.76 4.4 ± 1.83 27.9 ± 1.57 2.0 ± 0.53
G190S 52.0 ± 2.43 7.5 ± 1.28 37.6 ± 2.88 2.9 ± 0.94
a

Values are listed ± standard errors from the curve fit to the hyperbolic equation (see Materials and methods).