Table 4.
Steady state kinetics of single nucleotide incorporation
| RTs | Template-primer
|
|
|---|---|---|
| RNA–DNA kcat (s−1) | DNA–DNA kcat (s−1) | |
| WT | 0.060 ± 0.0192 | 0.019 ± 0.0300 |
| K103N | 0.049 ± 0.0223 | 0.042 ± 0.0065 |
| G190A | 0.035 ± 0.0038 | 0.046 ± 0.0208 |
| G190S | 0.021 ± 0.0148 | 0.023 ± 0.0111 |
P = 0.066 for K103N kcat vs. wt, DNA/DNA template, t test.
P > 0.10 for all other mutants and templates, compared to wild-type, t test.