TABLE 3.
The concentration of metabolites in blood
| Fed |
Fasted |
|||||
|---|---|---|---|---|---|---|
| Wild type | PEPCK-Cmus | p value | Wild type | PEPCK-Cmus | p value | |
| Cholesterol (mg/dl) | 105.6 ± 12.7 (5) | 65.8 ± 10.2 (9) | 0.03a | 83.3 ± 8.2 (6) | 60.2 ± 8.7 (6) | 0.08 |
| Free fatty acid (mEq/liter) | 0.56 ± 0.09 (7) | 0.39 ± 0.06 (10) | 0.10 | 0.62 ± 0.23 (6) | 0.50 ± 0.10 (6) | 0.66 |
| Triglycerides (mg/dl) | 95.7 ± 17.7 (7) | 58.7 ± 7.5 (10) | 0.05a | 64.6 ± 22.3 (7) | 48.3 ± 8.0 (6) | 0.53 |
| Ketone bodies (mg/dl) | 1.91 ± 0.23 (7) | 2.82 ± 0.77 (10) | 0.35 | 6.2 ± 1.2 (6) | 6.3 ± 2.0 (6) | 0.96 |
| Glucose (mg/dl) | 150.7 ± 8.1 (3) | 217.7 ± 6.6 (3) | <0.01a | 119.0 ± 7.3 (5) | 136.4 ± 5.0 (9) | 0.09 |
| Blood urea nitrogen (mg/dl) | 29.3 ±2.9 (7) | 29.1 ± 1.8 (10) | 0.95 | 21.8 ± 4.6 (5) | 27.0 ± 2.8 (5) | 0.36 |
| Creatinine (mg/dl) | 0.11 ± 0.02 (7) | 0.16 ± 0.03 (10) | 0.22 | 0.12 ± 0.02 (5) | 0.13 ± 0.02 (6) | 0.66 |
| Creatine kinase (unit/liter) | 276.2 ± 108.5 (6) | 324.8 ± 69.0 (10) | 0.70 | 121.5 ± 13.1 (6) | 446.0 ± 130.2 (5) | 0.02a |
| Total bilirubin (mg/dl) | 0.27 ± 0.12 (7) | 0.11 ± 0.01 (10) | 0.13 | 0.76 ± 0.38 (5) | 0.10 ± 0.00 (2) | 0.35 |
| Total protein (g/dl) | 4.86 ± 0.20 (7) | 4.60 ± 0.07 (10) | 0.18 | 4.76 ± 0.14 (5) | 4.33 ± 0.44 (3) | 0.25 |
| Albumin (g/dl) | 2.73 ± 0.11 (7) | 2.93 ± 0.14 (10) | 0.32 | 3.06 ± 0.18 (5) | 2.53 ± 0.26 (3) | 0.14 |
a
Statistical significance.
PEPCK-Cmus mice and control animals were fed ad libitum or fasted for 18 h, and their blood was collected. The analysis of metabolites was performed by Marshfield Laboratories, as outlined under “Experimental Procedures.” The results are expressed as the means ± S.E. for the number of animals indicated in parentheses. The p value is calculated for PEPCK-Cmus mice and wild type animals.