Figure 2.

Toxicological assessment of HYA-PHMB-NC and polyhexanide.

Notes: MTS cell viability assay (A, B), crystal violet cell proliferation assay (C, D), and the IC₅₀ overview (E) after 24 hours (A, C) and 7 days (B, D) of exposure of HDMEC to the HYA-PHMB-NC show that the NC (lines and bars in dark gray) exhibit less toxic effects on the cells than polyhexanide solution (lines and bars in light gray) alone. n=3.

Abbreviations: PHMB, polyhexamethylene biguanide; HYA-PHMB-NC, hyaluronan nanocapsules containing polyhexamethylene biguanide; HDMEC, human dermal microvascular endothelial cell; NC, nanocapsule; IC₅₀, half-maximal inhibitory concentration; c(polyhexanide); concentration of polyhexanide.