Fig. 2.

Analysis of apoptosis induction and cell proliferation. (a) Caspase 3/7 assay. Jurkat A3 cells were treated with indicated amounts of APG293 or TRAIL to induce apoptosis. Dose-dependent caspase activation was measured after 4 h of incubation. (b) APG101 or APG122 were used to compete with the proapoptotic activity of APG293 or TRAIL. 250 ng/ml APG293 or TRAIL were coincubated with increasing amounts of APG101 or APG122, respectively, and then added to Jurkat A3 cells. Dose-dependent decrease in caspase activation was measured after 4 h of incubation. (c, d) MTS-based cell viability assay: indicated cell lines were exposed to APG293 (1–1000 ng/ml) and APG101 (16–500 μg/ml) for 48 h before the addition of MTS.