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. 2015 Jun 6;26(7):716–727. doi: 10.1097/CAD.0000000000000237

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Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.

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Fig. 3 — Enzyme-linked immunosorbent assay (ELISA)-based analysis of CD95L-knockdown efficacy. (a) ELISA-based verification of the CD95L knockdown in U87-MG and U251-MG cells. Whole-cell lysates of cells transfected with sh[SCRMBL] or sh[CD95L] vector containing 50 μg of total protein were used to compare the abundance of endogenous CD95L. The calculated amount of CD95L and LOD was derived from a calibration curve using APG293 as the control compound. (b) ELISA-based analysis of CD95 upon knockdown of CD95L. The calculated amount of CD95 was derived from a calibration curve using APG101 as the control compound.