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. 2015 Jun 6;26(7):716–727. doi: 10.1097/CAD.0000000000000237

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Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.

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Fig. 4 — Spheroid invasion assay. (a) Matrigel-based three-dimensional spheroid invasion assay using spheroids of transfected U87-MG cells shown at the start of the assay and after 72 h. Spheroids of the control-transfected cell line U87[SCRMBL] showed invasive behaviour in vitro without stimulation by APG293, whereas their untreated counterparts after CD95L knockdown were refractory. (b) Invasion of U87[CD95L] knockdown cells was induced by the addition of 5 ng/ml APG293 to the semisolid assay matrix. Coincubation of APG293 was performed for 30 min with increasing amounts of APG101. Final concentrations of compounds are indicated in the figure. Spheroids are shown at the start of the assay (0 h) and after 72 h.